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Image Search Results
Journal: Journal of Cardiovascular Development and Disease
Article Title: The CCR2 + Monocyte Subsets Increase in Obese Boys but Not Girls with Abnormally High Carotid Intima-Media Thickness: A Pilot Study
doi: 10.3390/jcdd9100330
Figure Lengend Snippet: Gating Strategy. We first gated total leukocytes on a time/side scatter density plot and selected the Zombie UV negative cell population for detecting living cells. Next, we gated living cells for singlets on a forward scatter/trigger pulse width density plot. After recognizing cells by size and granularity, we selected monocytes on the HLA-DR gate. Then, we gated monocytes using the rectangular gating strategy on the CD14 + /CD16 + cell population to identify CD14 ++ CD16 − cells as classical monocytes, CD14 ++ CD16 + cells as intermediate monocytes, and CD14 + CD16 + cells as nonclassical monocytes . We obtained the median fluorescence intensity (MFI) for CCR2 by considering both positive and negative cell populations. We got the CCR2 + cell percentage using fluorescence minus one (FMO) control. For each fluorochrome, we used compensation controls through UltraComp eBeads TM (Invitrogen TM , Carlsbad, CA, USA). We analyzed data by the FlowJo 10.0.7 software (TreeStar, Inc., Ashland, OR, USA).
Article Snippet: We analyzed monocyte subpopulations using the
Techniques: Fluorescence, Software